PCR primer search script
Required skills: Biology, Biotechnology, Python
Program that searches PCR primers in a given dna sequence in Python
in the 5' - 3' strand and the reverse complement strand
No need for GUI
programs searches and gives the first hit as output for each strand
Primer is a piece of dna that has:
Length of 17 to 30 bases
GC-content about 50-60% (if possible)
The 3' end should have a G or C as the end base
Tm (melting temperature) between 55 and 65 ºC
Tm = 2ºC(A+T) + 4ºC (C+G) (A, T, C, G - number of respective bases)
Input:
Fasta file, or copy paste DNA sequence
output program:
Sequence primer 1 from 5' - 3':
Sequence primer 2 from 5' - 3':
in the 5' - 3' strand and the reverse complement strand
No need for GUI
programs searches and gives the first hit as output for each strand
Primer is a piece of dna that has:
Length of 17 to 30 bases
GC-content about 50-60% (if possible)
The 3' end should have a G or C as the end base
Tm (melting temperature) between 55 and 65 ºC
Tm = 2ºC(A+T) + 4ºC (C+G) (A, T, C, G - number of respective bases)
Input:
Fasta file, or copy paste DNA sequence
output program:
Sequence primer 1 from 5' - 3':
Sequence primer 2 from 5' - 3':
Posted In:PythonBiologyBiotechnology |
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